Sub-Zero may refer to:
- Bi-Han, the series' first Sub-Zero who debut in the original Mortal Kombat. He is later killed by Hanzo Hasashi and resurrected as the Wraith Noob Saibot in the original and second timelines before returning to the Sub-Zero name in the new timeline.
- Kuai Liang, the series' second Sub-Zero and the younger brother of Bi-Han. Under the codename Tundra, he takes the mantle of Sub-Zero to honor his brother in the original and second timelines.
- Sub-Zero, the first Sub-Zero and the grandfather of Bi-Han and Kuai Liang, from Mortal Kombat: Deception.
- Sub-Zero, a character in the series Mortal Kombat: Conquest, which is non-canon to the Mortal Kombat games.